How much bacteriostatic water do I add to 5mg of MOTS-c?

The standard protocol is to add 2 ml of bacteriostatic water to a 5 mg vial of MOTS-c. This produces a concentration of 2.50 mg/ml (2500 mcg/ml). Adjust the water volume down if you want a more concentrated solution for smaller syringe volumes, or up if you prefer easier-to-read markings.

What is the standard dose of MOTS-c after reconstitution?

A common MOTS-c research dose is 5.0 mg per administration. At a concentration of 2.50 mg/ml, this equals approximately 200.0 IU on a 100-unit insulin syringe (2.000 ml per dose). Always confirm your dose against your protocol — this is a starting reference only.

How long does reconstituted MOTS-c last in the fridge?

Reconstituted MOTS-c in bacteriostatic water typically remains stable for 28 days refrigerated at 2–8°C (36–46°F). BAC water contains 0.9% benzyl alcohol as a preservative. Never freeze, never leave at room temperature, and discard if the solution becomes cloudy or discolored.

What concentration does 5mg MOTS-c in 2ml water give?

5 mg of MOTS-c reconstituted in 2 ml of BAC water produces a concentration of 2.50 mg/ml, which equals 2500 mcg/ml. This vial yields roughly 1 full doses at 5000 mcg per administration.

Can I use sterile water instead of BAC water for MOTS-c?

You can, but it is not recommended for multi-dose vials. Sterile water has no benzyl alcohol preservative, so reconstituted MOTS-c must be used within 24 hours. BAC water allows the vial to last 28 days refrigerated. Use sterile water only if you plan to finish the entire vial in a single session.

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How to Reconstitute MOTS-c

Pre-filled calculator with standard defaults for MOTS-c: 5 mg vial in 2 ml BAC water.

Standard MOTS-c Reconstitution Protocol

Add 2 ml of bacteriostatic water to a 5 mg vial of MOTS-c. This gives a concentration of 2.50 mg/ml. A standard 5000 mcg dose equals about 200.0 IU on a 100-unit insulin syringe. Store refrigerated at 2–8°C and use within 28 days.

Vial Size

BAC Water

Desired Dose

mcg

Concentration

2.50

mg/ml

2500 mcg/ml

Dose Volume

2.000

Per dose

Syringe Draw

200.0

100-unit insulin syringe

Doses per Vial

doses

1.0 exact

Draw to

100

100.0 units

Draw to this line

Step-by-Step: How to Reconstitute MOTS-c

1

Gather your supplies

A sealed 5 mg vial of MOTS-c, a vial of bacteriostatic water, a 1 ml (100-unit) insulin syringe, and an alcohol swab.
2

Inject the water

Wipe both vial stoppers with alcohol. Draw 2 ml of BAC water into the syringe and inject it slowly down the inside wall of the MOTS-c vial. Never spray directly onto the powder — the force can damage the peptide.
3

Swirl gently

Gently swirl the vial in a circular motion until the MOTS-c fully dissolves. Do not shake. The solution should become clear.
4

Calculate your dose

At a concentration of 2.50 mg/ml, a 5000 mcg (5.000 mg) dose equals about 200.0 IU on a 100-unit insulin syringe.
5

Draw and store

Draw your dose, perform the injection, and immediately return the vial to the refrigerator at 2–8°C. Use the reconstituted vial within 28 days.

These defaults reflect the most common community protocols for MOTS-c. Individual research protocols may differ. Always verify against your source and consult a qualified practitioner before any injection. BodyHackGuide publishes for research and educational purposes only.

MOTS-c Reconstitution FAQ

More MOTS-c Resources

MOTS-c compound page MOTS-c dosage guide General reconstitution calculator Verify your MOTS-c COA

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