How much bacteriostatic water do I add to 5mg of GHK-Cu?

The standard protocol is to add 2 ml of bacteriostatic water to a 5 mg vial of GHK-Cu. This produces a concentration of 2.50 mg/ml (2500 mcg/ml). Adjust the water volume down if you want a more concentrated solution for smaller syringe volumes, or up if you prefer easier-to-read markings.

What is the standard dose of GHK-Cu after reconstitution?

A common GHK-Cu research dose is 200 mcg per administration. At a concentration of 2.50 mg/ml, this equals approximately 8.0 IU on a 100-unit insulin syringe (0.080 ml per dose). Always confirm your dose against your protocol — this is a starting reference only.

How long does reconstituted GHK-Cu last in the fridge?

Reconstituted GHK-Cu in bacteriostatic water typically remains stable for 14 days refrigerated at 2–8°C (36–46°F). BAC water contains 0.9% benzyl alcohol as a preservative. Never freeze, never leave at room temperature, and discard if the solution becomes cloudy or discolored.

What concentration does 5mg GHK-Cu in 2ml water give?

5 mg of GHK-Cu reconstituted in 2 ml of BAC water produces a concentration of 2.50 mg/ml, which equals 2500 mcg/ml. This vial yields roughly 25 full doses at 200 mcg per administration.

Can I use sterile water instead of BAC water for GHK-Cu?

You can, but it is not recommended for multi-dose vials. Sterile water has no benzyl alcohol preservative, so reconstituted GHK-Cu must be used within 24 hours. BAC water allows the vial to last 14 days refrigerated. Use sterile water only if you plan to finish the entire vial in a single session.

How much bacteriostatic water do I add to 5mg of GHK-Cu?

The standard protocol is to add 2 ml of bacteriostatic water to a 5 mg vial of GHK-Cu. This produces a concentration of 2.50 mg/ml (2500 mcg/ml). Adjust the water volume down if you want a more concentrated solution for smaller syringe volumes, or up if you prefer easier-to-read markings.

What is the standard dose of GHK-Cu after reconstitution?

A common GHK-Cu research dose is 200 mcg per administration. At a concentration of 2.50 mg/ml, this equals approximately 8.0 IU on a 100-unit insulin syringe (0.080 ml per dose). Always confirm your dose against your protocol — this is a starting reference only.

How long does reconstituted GHK-Cu last in the fridge?

Reconstituted GHK-Cu in bacteriostatic water typically remains stable for 14 days refrigerated at 2–8°C (36–46°F). BAC water contains 0.9% benzyl alcohol as a preservative. Never freeze, never leave at room temperature, and discard if the solution becomes cloudy or discolored.

What concentration does 5mg GHK-Cu in 2ml water give?

5 mg of GHK-Cu reconstituted in 2 ml of BAC water produces a concentration of 2.50 mg/ml, which equals 2500 mcg/ml. This vial yields roughly 25 full doses at 200 mcg per administration.

Can I use sterile water instead of BAC water for GHK-Cu?

You can, but it is not recommended for multi-dose vials. Sterile water has no benzyl alcohol preservative, so reconstituted GHK-Cu must be used within 24 hours. BAC water allows the vial to last 14 days refrigerated. Use sterile water only if you plan to finish the entire vial in a single session.

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How to Reconstitute GHK-Cu

Pre-filled calculator with standard defaults for GHK-Cu: 5 mg vial in 2 ml BAC water.

Standard GHK-Cu Reconstitution Protocol

Add 2 ml of bacteriostatic water to a 5 mg vial of GHK-Cu. This gives a concentration of 2.50 mg/ml. A standard 200 mcg dose equals about 8.0 IU on a 100-unit insulin syringe. Store refrigerated at 2–8°C and use within 14 days.

Vial Size

BAC Water

Desired Dose

mcg

Concentration

2.50

mg/ml

2500 mcg/ml

Dose Volume

0.080

Per dose

Syringe Draw

8.0

100-unit insulin syringe

Doses per Vial

doses

25.0 exact

Draw to

100

8.0 units

Draw to this line

GHK-Cu Reconstitution Chart

Common syringe draws at the standard GHK-Cu preset (5 mg in 2 mL BAC water = 2.50 mg/mL). Units are on a U-100 insulin syringe (100 units = 1 mL). For laboratory research reference only.

Syringe units (U-100)	Volume	Amount (GHK-Cu)
5 units	0.05 mL	125 mcg
10 units	0.10 mL	250 mcg
15 units	0.15 mL	375 mcg
20 units	0.20 mL	500 mcg
25 units	0.25 mL	625 mcg
30 units	0.30 mL	750 mcg
40 units	0.40 mL	1.00 mg
50 units	0.50 mL	1.25 mg

Change the vial size or BAC water above to recompute the chart for a different GHK-Cu preset. This chart shows reconstitution math, not a dosing recommendation.

Step-by-Step: How to Reconstitute GHK-Cu

1

Gather your supplies

A sealed 5 mg vial of GHK-Cu, a vial of bacteriostatic water, a 1 ml (100-unit) insulin syringe, and an alcohol swab.
2

Inject the water

Wipe both vial stoppers with alcohol. Draw 2 ml of BAC water into the syringe and inject it slowly down the inside wall of the GHK-Cu vial. Never spray directly onto the powder — the force can damage the peptide.
3

Swirl gently

Gently swirl the vial in a circular motion until the GHK-Cu fully dissolves. Do not shake. The solution should become clear.
4

Calculate your dose

At a concentration of 2.50 mg/ml, a 200 mcg (0.200 mg) dose equals about 8.0 IU on a 100-unit insulin syringe.
5

Draw and store

Draw your dose, perform the injection, and immediately return the vial to the refrigerator at 2–8°C. Use the reconstituted vial within 14 days.

These defaults reflect the most common community protocols for GHK-Cu. Individual research protocols may differ. Always verify against your source and consult a qualified practitioner before any injection. BodyHackGuide publishes for research and educational purposes only.

GHK-Cu Reconstitution FAQ

More GHK-Cu Resources

GHK-Cu compound page GHK-Cu dosage guide General reconstitution calculator Verify your GHK-Cu COA

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