How much bacteriostatic water do I add to 5mg of GHK-Cu?

The standard protocol is to add 2 ml of bacteriostatic water to a 5 mg vial of GHK-Cu. This produces a concentration of 2.50 mg/ml (2500 mcg/ml). Adjust the water volume down if you want a more concentrated solution for smaller syringe volumes, or up if you prefer easier-to-read markings.

What is the standard dose of GHK-Cu after reconstitution?

A common GHK-Cu research dose is 200 mcg per administration. At a concentration of 2.50 mg/ml, this equals approximately 8.0 IU on a 100-unit insulin syringe (0.080 ml per dose). Always confirm your dose against your protocol — this is a starting reference only.

How long does reconstituted GHK-Cu last in the fridge?

Reconstituted GHK-Cu in bacteriostatic water typically remains stable for 14 days refrigerated at 2–8°C (36–46°F). BAC water contains 0.9% benzyl alcohol as a preservative. Never freeze, never leave at room temperature, and discard if the solution becomes cloudy or discolored.

What concentration does 5mg GHK-Cu in 2ml water give?

5 mg of GHK-Cu reconstituted in 2 ml of BAC water produces a concentration of 2.50 mg/ml, which equals 2500 mcg/ml. This vial yields roughly 25 full doses at 200 mcg per administration.

Can I use sterile water instead of BAC water for GHK-Cu?

You can, but it is not recommended for multi-dose vials. Sterile water has no benzyl alcohol preservative, so reconstituted GHK-Cu must be used within 24 hours. BAC water allows the vial to last 14 days refrigerated. Use sterile water only if you plan to finish the entire vial in a single session.

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How to Reconstitute GHK-Cu

Pre-filled calculator with standard defaults for GHK-Cu: 5 mg vial in 2 ml BAC water.

Standard GHK-Cu Reconstitution Protocol

Add 2 ml of bacteriostatic water to a 5 mg vial of GHK-Cu. This gives a concentration of 2.50 mg/ml. A standard 200 mcg dose equals about 8.0 IU on a 100-unit insulin syringe. Store refrigerated at 2–8°C and use within 14 days.

Vial Size

BAC Water

Desired Dose

mcg

Concentration

2.50

mg/ml

2500 mcg/ml

Dose Volume

0.080

Per dose

Syringe Draw

8.0

100-unit insulin syringe

Doses per Vial

doses

25.0 exact

Draw to

100

8.0 units

Draw to this line

Step-by-Step: How to Reconstitute GHK-Cu

1

Gather your supplies

A sealed 5 mg vial of GHK-Cu, a vial of bacteriostatic water, a 1 ml (100-unit) insulin syringe, and an alcohol swab.
2

Inject the water

Wipe both vial stoppers with alcohol. Draw 2 ml of BAC water into the syringe and inject it slowly down the inside wall of the GHK-Cu vial. Never spray directly onto the powder — the force can damage the peptide.
3

Swirl gently

Gently swirl the vial in a circular motion until the GHK-Cu fully dissolves. Do not shake. The solution should become clear.
4

Calculate your dose

At a concentration of 2.50 mg/ml, a 200 mcg (0.200 mg) dose equals about 8.0 IU on a 100-unit insulin syringe.
5

Draw and store

Draw your dose, perform the injection, and immediately return the vial to the refrigerator at 2–8°C. Use the reconstituted vial within 14 days.

These defaults reflect the most common community protocols for GHK-Cu. Individual research protocols may differ. Always verify against your source and consult a qualified practitioner before any injection. BodyHackGuide publishes for research and educational purposes only.

GHK-Cu Reconstitution FAQ

More GHK-Cu Resources

GHK-Cu compound page GHK-Cu dosage guide General reconstitution calculator Verify your GHK-Cu COA

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