How to Reconstitute Semaglutide
Pre-filled calculator with standard defaults for Semaglutide: 5 mg vial in 2 ml BAC water.
Standard Semaglutide Reconstitution Protocol
Add 2 ml of bacteriostatic water to a 5 mg vial of Semaglutide. This gives a concentration of 2.50 mg/ml. A 250 mcg amount measures about 10.0 IU on a U-100 syringe scale. Store refrigerated at 2–8°C and use within 56 days.
Concentration
2.50
mg/ml
2500 mcg/ml
Volume per Draw
0.100
ml
Per draw
Syringe Draw
10.0
IU
U-100 syringe scale
Draws per Vial
20
draws
20.0 exact
Draw to
10.0 units
Draw to this line
For laboratory research use only. This tool performs reconstitution math (concentration and syringe-unit conversion) and is not dosing guidance for humans or animals.
Semaglutide Reconstitution Chart
Common syringe draws at the standard Semaglutide preset (5 mg in 2 mL BAC water = 2.50 mg/mL). Units are on a U-100 syringe scale (100 units = 1 mL). For laboratory research reference only.
| Syringe units (U-100) | Volume | Amount (Semaglutide) |
|---|---|---|
| 5 units | 0.05 mL | 125 mcg |
| 10 units | 0.10 mL | 250 mcg |
| 15 units | 0.15 mL | 375 mcg |
| 20 units | 0.20 mL | 500 mcg |
| 25 units | 0.25 mL | 625 mcg |
| 30 units | 0.30 mL | 750 mcg |
| 40 units | 0.40 mL | 1.00 mg |
| 50 units | 0.50 mL | 1.25 mg |
Change the vial size or BAC water above to recompute the chart for a different Semaglutide preset. This chart shows reconstitution math, not a usage recommendation.
Step-by-Step: How to Reconstitute Semaglutide
-
1
Gather your supplies
A sealed 5 mg vial of Semaglutide, a vial of bacteriostatic water, a 1 ml U-100 syringe, and an alcohol swab.
-
2
Add the water
Wipe both vial stoppers with alcohol. Draw 2 ml of BAC water into the syringe and release it slowly down the inside wall of the Semaglutide vial. Never spray directly onto the powder — the force can damage the peptide.
-
3
Swirl gently
Gently swirl the vial in a circular motion until the Semaglutide fully dissolves. Do not shake. The solution should become clear.
-
4
Calculate the amount
At a concentration of 2.50 mg/ml, a 250 mcg (0.250 mg) amount equals about 10.0 IU on a U-100 syringe scale.
-
5
Draw and store
Draw the amount you need, then immediately return the vial to the refrigerator at 2–8°C. Use the reconstituted vial within 56 days.
These defaults reflect commonly referenced reconstitution presets for Semaglutide. Individual research setups may differ. Always verify the concentration against your source and its certificate of analysis. BodyHackGuide publishes for laboratory research and educational purposes only.