--- title: "How to Reconstitute Peptides: Step-by-Step Guide" url: https://www.bodyhackguide.co/guides/reconstitution-guide description: "The complete walkthrough for reconstituting lyophilized peptides with bacteriostatic water โ€” no guesswork needed." lang: en --- Skip to content Compare notes on r/BodyHackGuide (https://reddit.com/r/bodyhackguide) Compare notes with other researchers. Join r/BodyHackGuide (https://reddit.com/r/bodyhackguide) All Guides (https://www.bodyhackguide.co/guides) ๐Ÿ’‰ # How to Reconstitute Peptides: Step-by-Step Guide The complete walkthrough for reconstituting lyophilized peptides with bacteriostatic water โ€” no guesswork needed. getting-started 2 min read Updated 3/10/2026 ## What You Need - Your lyophilized peptide vial - Bacteriostatic water (BAC water) โ€” NOT sterile water, NOT saline - Insulin syringes (typically 1mL / 100 unit) - Alcohol swabs - A clean workspace ## Why Bacteriostatic Water? BAC water contains 0.9% benzyl alcohol, which inhibits bacterial growth. This means your reconstituted peptide stays viable for weeks in the fridge. Regular sterile water has no preservative โ€” once you open it, bacteria can grow. ## Step-by-Step Process ### 1. Prep Your Workspace Clean surface, wash hands. Swab the tops of both the peptide vial and BAC water vial with alcohol pads. ### 2. Draw Your BAC Water Decide how much water to add. Common amounts: - **1mL** into a 5mg vial = 5mg/mL (each 0.1mL = 500mcg) - **2mL** into a 10mg vial = 5mg/mL (each 0.1mL = 500mcg) Use our Reconstitution Calculator (https://www.bodyhackguide.co/tools/reconstitution) to figure out exact dosing. ### 3. Add Water to Peptide Vial **Critical:** Aim the stream of water down the SIDE of the vial, not directly onto the powder. Let it run down the glass wall gently. Never shake the vial. ### 4. Let It Dissolve Gently swirl if needed. Most peptides dissolve within a few minutes. If it's not dissolving, let it sit in the fridge for 15-30 minutes. Some peptides take longer. ### 5. Storage - **Refrigerate** at 2-8ยฐC (standard fridge) - Keep away from light - Use within 4 weeks typically - Never freeze reconstituted peptides ## Reading Your Syringe A standard 1mL insulin syringe has 100 units: - 10 units = 0.1mL - 5 units = 0.05mL If you reconstituted 10mg in 2mL, then each unit on the syringe = 50mcg. Use our Dosage Calculator (https://www.bodyhackguide.co/tools/dosage) to convert. ## Pro Tips - Label your vials with the compound name, concentration, and date reconstituted - Pre-load syringes for convenience, but use within 24 hours if you do - If the solution is cloudy or has particles, don't use it - BAC water vials can be reused โ€” just always swab the top ## Related Guides ๐Ÿงฌ getting-started ### Peptide Research 101: What You Need to Know Before You Start A no-BS intro to peptide research โ€” what they are, how they work, and what to watch out for as a beginner. Read https://www.bodyhackguide.co/guides/peptide-research-101 โ† Back to all guides: https://www.bodyhackguide.co/guides Image: BodyHackGuide (https://www.bodyhackguide.co/assets/bodyhackguide-logo-DOzZNUyh.webp) ### BodyHackGuide Your biohacking research hub: compound pricing, brain health protocols, dosing tools, and vendor reviews. support@bodyhackguide.co Join our Discord: https://discord.gg/Mhq5UdRYBA r/BodyHackGuide on Reddit: https://reddit.com/r/BodyHackGuide #### Explore - Compare prices: https://www.bodyhackguide.co/compare - Vendors: https://www.bodyhackguide.co/vendors - Deals: https://www.bodyhackguide.co/deals - Coupons: https://www.bodyhackguide.co/coupons - Nootropics: https://www.bodyhackguide.co/nootropics - Brain health: https://www.bodyhackguide.co/brain-health - Blog: https://www.bodyhackguide.co/blog - Guides: https://www.bodyhackguide.co/guides #### Tools - Reconstitution: https://www.bodyhackguide.co/tools/reconstitution - Half-life visualizer: https://www.bodyhackguide.co/tools/halflife - COA reader: https://www.bodyhackguide.co/tools/coa - Cost calculator: https://www.bodyhackguide.co/tools/cost-calculator - Stack builder: https://www.bodyhackguide.co/stack-builder - All tools: https://www.bodyhackguide.co/tools #### Trust & vendors - Vendor directory: https://www.bodyhackguide.co/vendors - Trust scorecard: https://www.bodyhackguide.co/vendors/scorecard - Vendor trust badge: https://www.bodyhackguide.co/vendors/get-vetted - Scoring methodology: https://www.bodyhackguide.co/vendors/methodology - Peptide cheat sheet: https://www.bodyhackguide.co/guides/peptide-cheat-sheet-download #### Company - About: https://www.bodyhackguide.co/about - Affiliate disclosure: https://www.bodyhackguide.co/affiliate-disclosure - Editorial standards: https://www.bodyhackguide.co/editorial-standards - Creators: https://www.bodyhackguide.co/creators - Coaches: https://www.bodyhackguide.co/coaches - 1-on-1 coaching: https://www.bodyhackguide.co/coaching - Privacy: https://www.bodyhackguide.co/privacy - Terms: https://www.bodyhackguide.co/terms - Full site directory: https://www.bodyhackguide.co/site-directory **Affiliate Disclosure:** We rank every vendor on the same public, reproducible methodology (https://www.bodyhackguide.co/vendors/methodology), and we earn a commission from purchases made through some of our links at no extra cost to you. 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