--- title: "TB-500: Dosing & Vendor Prices | BodyHackGuide" url: https://www.bodyhackguide.co/compound/tb-500 description: "TB-500: dosing protocols, mechanism & side effects, 1,039 PubMed results. Compare 4 current vendor prices." lang: en --- Skip to content Compare notes on r/BodyHackGuide (https://reddit.com/r/bodyhackguide) Compare notes with other researchers. Join r/BodyHackGuide (https://reddit.com/r/bodyhackguide) Image: TB-500 molecular structure (https://www.bodyhackguide.co/assets/peptide-structure-placeholder-DUmZBF_j.png) # TB-500 Injury, Repair & Recovery (https://www.bodyhackguide.co/wiki#cat-injury,-repair-&-recovery)Phase 2 Also known as: Thymosin Beta-4 fragment (17-23), TB4 fragment, TB500, TB 500, Ac-LKKTETQ TB-500 is the name the market and the FDA use for a short synthetic fragment of thymosin beta-4: the seven-amino-acid, N-acetylated actin-binding sequence Ac-LKKTETQ (residues 17 to 23). Thymosin beta-4 itself is a 43-amino-acid peptide (about 4963 Da acetylated) and one of the most abundant intracellular actin-sequestering molecules in mammalian cells. Image: Ion Peptide logo (https://ionpeptide.com/wp-content/uploads/2025/08/ion-peptide-logo-1-300x148.jpg) Featured vendor $5.80/mg $29.00 for 5mg at Ion Peptide · Buy at Ion Peptide: https://ionpeptide.com/product/tb-500/?ref=reddit15 Half-life: Plasma half-life is short - full-length thymosin beta-4 clear… Route: Subcutaneous, Intramuscular MW: 889 Da (Ac-LKKTETQ) CAS: 77591-33-4 1,039 PubMed results (https://pubmed.ncbi.nlm.nih.gov/?term=thymosin%20beta-4) Last reviewed: May 4, 2026 Reconstitution Calculator for TB-500 Pre-filled · 5mg vial · 2500mcg dose https://www.bodyhackguide.co/tools/reconstitution/tb-500 ## Overview ### At A Glance Mechanism Actin Sequestration and Cytoskeletal Dynamics… Half-Life Plasma half-life is short - full-length thymosin beta-4 clears on the order of hours, while the short LKKTETQ actin-binding fragment clears within minutes. Tissue-level regenerative effects persist substantially longer than plasma exposure. Dosing 2x per week during loading; 1x per week maintenance Dose Range 2000-5000 mcg (2-5 mg) subcutaneous 2x weekly loading; 2000 mcg (2 mg) weekly maintenance Routes Subcutaneous Intramuscular Common Vials 2mg 5mg 10mg Potential Benefits Accelerated dermal wound healing through enhanced keratinocyte and fibroblast migration, collagen deposition, and angiogenesis, including in diabetic and aged models (PMID: 12581423) Cardioprotection and tissue repair after myocardial infarction via ILK/Akt survival signaling in preclinical models (PMID: 17600280) Anti-inflammatory effects via NF-kB suppression and reduced pro-inflammatory cytokines (preclinical) Corneal wound healing and dry eye symptom improvement in topical ophthalmic (RGN-259) Phase 2 trials (PMID: 25826322) Enhanced hair follicle stem cell migration associated with hair growth (preclinical) Musculoskeletal recovery through collagen deposition and matrix remodeling (preclinical and equine data) Endothelial cell migration and vascular regeneration in ischemic tissues (preclinical) Safety Notes Common Injection site discomfort (subcutaneous administration) Mild fatigue or lethargy in the days following initial doses Temporary head rush or flushing reported anecdotally Serious No serious adverse events in published preclinical literature Theoretical risk of promoting growth of existing tumors due to angiogenic properties Limited human safety data — most evidence from animal models and equine use ### Overview TB-500 is the name the market and the FDA use for a short synthetic fragment of thymosin beta-4: the seven-amino-acid, N-acetylated actin-binding sequence Ac-LKKTETQ (residues 17 to 23). Thymosin beta-4 itself is a 43-amino-acid peptide (about 4963 Da acetylated) and one of the most abundant intracellular actin-sequestering molecules in mammalian cells. The two are not the same molecule. Analytical work on internet-sold TB-500 found products that did not match their descriptions (PMID: 36482504), and a 2024 metabolite study states that the biological effects of the acetylated TB-500 fragment have not been documented (PMID: 38382158). The sequence and mass on a lot certificate of analysis are the only way to know which form a vial holds. The foundational review by Goldstein et al. (2005) established full-length thymosin beta-4 as a multifunctional regenerative peptide with roles spanning wound healing, angiogenesis, anti-inflammation, and cardiac repair. Unlike many bioactive peptides, thymosin beta-4 is not a hormone or cytokine but rather an intracellular actin-regulatory protein that, when released extracellularly following injury, initiates paracrine signaling cascades that drive tissue repair. Most of what follows is full-length thymosin beta-4 data; the fragment's efficacy data appears only in the wound-healing paragraph. Thymosin beta-4 has been studied extensively in preclinical models of cardiac injury. Bock-Marquette et al. (2004) demonstrated that thymosin beta-4 promotes survival of cardiomyocytes after experimental myocardial infarction in mice, reduces infarct size, and improves cardiac function. The mechanism involves activation of the Akt (protein kinase B) survival pathway and migration of cardiomyocytes and endothelial cells toward the injury. These findings positioned thymosin beta-4 as a candidate cardiac repair agent, though clinical translation has been limited. In wound healing research, thymosin beta-4 accelerates dermal wound closure by promoting keratinocyte and endothelial cell migration, increasing angiogenesis, and reducing inflammation at the wound site. Philp et al. (2003) showed that topical thymosin beta-4 significantly accelerated full-thickness wound closure in diabetic and aged mouse models, and in the same paper the free seven-residue fragment LKKTETQ promoted repair in aged animals comparable to the parent peptide (PMID: 12581423). That one mouse study, plus in-vitro assays, is the fragment record; there is no human data for the fragment. Thymosin beta-4 has also shown efficacy in corneal wound healing models. Sosne et al. demonstrated that thymosin beta-4 eye drops accelerate corneal epithelial wound closure and reduce inflammation after chemical or mechanical injury, leading to ophthalmic clinical trials (Phase 2 and Phase 3, RGN-259) for dry eye and neurotrophic keratopathy. This is the most advanced clinical development program for any thymosin beta-4-based therapeutic, and it is topical. TB-500 is not FDA-approved for any human therapeutic indication. It is a doping-control target in racing horses (PMID: 23084823) and is used in the human peptide biohacking community for musculoskeletal recovery, wound healing, and anti-inflammatory effects, on the strength of the full-length peptide literature rather than any human fragment data. ### Potential Research Fields Cardiac repair Wound healing Dry eye syndrome Corneal healing Muscle injury ## Chemical Information IUPAC Name N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-threonyl-L-alpha-glutamyl-L-threonyl-L-glutamine CAS Number 77591-33-4 Molecular Formula C38H68N10O14 (Ac-LKKTETQ); full-length thymosin beta-4 is C212H350N56O78S Molecular Mass 889.0 g/mol (Ac-LKKTETQ); 846.97 g/mol (LKKTETQ, free); full-length thymosin beta-4 4963.4 g/mol acetylated Image: TB-500 molecular structure (https://pubchem.ncbi.nlm.nih.gov/rest/pug/compound/cid/16132380/PNG?image_size=300x300) View on PubChem: https://pubchem.ncbi.nlm.nih.gov/compound/16132380 Amino Acid Sequence Ac-LKKTETQ (thymosin beta-4 residues 17-23). Full-length thymosin beta-4 for comparison: Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES ## Dosing & Protocols ### Unlock the dosing protocols Enter your email to keep reading. It is free, and it opens these tabs on every compound page in this browser. - Dose, route, how often and how long - A titration schedule - Beginner, intermediate and advanced protocols - Reconstitution and handling notes You also get the free peptide cheat sheet by email. Unsubscribe anytime. Have an account? Sign in (https://www.bodyhackguide.co/auth) ## Research ### Unlock the research summary - A summary of the key research - Safety and side effects - A link to the PubMed results ## Interactions ### Interaction Matrix ### Contraindications Active cancer (theoretical concern with tissue growth promotion). Pregnancy or breastfeeding. Known hypersensitivity to thymosin beta-4 fragments. Research Disclaimer This interaction data is compiled from published research and community reports. It may not be exhaustive. Always consult a healthcare professional before combining compounds. Best Price $29.00 up to $64.99 Best $/mg $4.9000 Vendors 4 Listings 8 vial Dosage Form Sort | Vendor | Product | Form | Qty | Price | $/mg | Coupon | | | --- | --- | --- | --- | --- | --- | --- | --- | | Image: Disguised Alpha logo (https://disguisedalpha.com/wp-content/themes/assets/logos/disguised-logo-2x.png) Disguised Alpha (https://www.bodyhackguide.co/vendors/disguised-alpha) 50: https://www.bodyhackguide.co/vendor-trust-scorecard 🇺🇸 US 🇪🇺 EU 🇬🇧 UK | TB-500 (vial) | vial | 1 vial ● In Stock | $64.99 | — | — | Buy: https://disguisedalpha.com/product/tb-4/?coupon=reddit | | Image: Ion Peptide logo (https://ionpeptide.com/wp-content/uploads/2025/08/ion-peptide-logo-1-300x148.jpg) Ion Peptide (https://www.bodyhackguide.co/vendors/ion-peptide) 70: https://www.bodyhackguide.co/vendor-trust-scorecard 🇺🇸 US 🌍 International | TB-500 5mg | vial | 1 vial ● In Stock | $29.00 BEST | $5.80 | — | Buy: https://ionpeptide.com/product/tb-500/?ref=reddit15 | | Image: Ion Peptide logo (https://ionpeptide.com/wp-content/uploads/2025/08/ion-peptide-logo-1-300x148.jpg) Ion Peptide (https://www.bodyhackguide.co/vendors/ion-peptide) 70: https://www.bodyhackguide.co/vendor-trust-scorecard 🇺🇸 US 🌍 International | TB-500 10mg | vial | 1 vial ● In Stock | $49.00 VALUE | $4.90 | — | Buy: https://ionpeptide.com/product/tb-500/?ref=reddit15 | | Image: Ion Peptide logo (https://ionpeptide.com/wp-content/uploads/2025/08/ion-peptide-logo-1-300x148.jpg) Ion Peptide (https://www.bodyhackguide.co/vendors/ion-peptide) 70: https://www.bodyhackguide.co/vendor-trust-scorecard 🇺🇸 US 🌍 International | TB-500 20mg | vial | 1 vial ● Out of Stock | $89.00 | $4.45 | — | View: https://ionpeptide.com/product/tb-500/?ref=reddit15 | | Image: BioMyst Labs logo (https://biomystlabs.com/wp-content/uploads/2025/12/Asset-36@3x-scaled.png) BioMyst Labs (https://www.bodyhackguide.co/vendors/biomyst-labs) 70: https://www.bodyhackguide.co/vendor-trust-scorecard 🇺🇸 US 🌍 International | TB-500 5mg | vial | 1 vial ● In Stock | $34.99 | $7.00 | — | Buy: https://biomystlabs.com/product/tb500-5mg/?ref=rxtkqorwv | | Image: BioMyst Labs logo (https://biomystlabs.com/wp-content/uploads/2025/12/Asset-36@3x-scaled.png) BioMyst Labs (https://www.bodyhackguide.co/vendors/biomyst-labs) 70: https://www.bodyhackguide.co/vendor-trust-scorecard 🇺🇸 US 🌍 International | TB-500 5mg | vial | 1 vial ● In Stock | $36.99 | $7.40 | — | Buy: https://biomystlabs.com/product/tb500-5mg/?ref=rxtkqorwv | | Image: VANDL Labs logo (https://halfnattys.shop/wp-content/themes/halfnattys/assets/img/logo.webp) VANDL Labs (https://www.bodyhackguide.co/vendors/vandl-labs) 50: https://www.bodyhackguide.co/vendor-trust-scorecard 🇺🇸 US | TB-500 5mg | vial | 5mg vial ● In Stock | $34.99 | $7.00 | CHON | Buy: https://www.vandl-labs.com/product/tb-500/?ref=choncho | | Image: VANDL Labs logo (https://halfnattys.shop/wp-content/themes/halfnattys/assets/img/logo.webp) VANDL Labs (https://www.bodyhackguide.co/vendors/vandl-labs) 50: https://www.bodyhackguide.co/vendor-trust-scorecard 🇺🇸 US | TB-500 10mg | vial | 10mg vial ● In Stock | $49.99 | $5.00 | CHON | Buy: https://www.vandl-labs.com/product/tb-500/?ref=choncho | ### Sign in to leave a review Reviews on BodyHackGuide are tied to verified user accounts and moderated before publishing. Sign in (free, no spam) to share your experience with TB-500. Current low $29.00 current listings 7-day low — not enough history yet 30-day low — not enough history yet 30-day change — not enough history yet Tracking since Mar 13, 2026 · 8 data points ## Price History 4 data points ### Vendors Selling TB-500 #### Ion Peptide 4.5 3 listings · from $29.00 https://www.bodyhackguide.co/vendors/ion-peptide #### BioMyst Labs 4.5 2 listings · from $34.99 https://www.bodyhackguide.co/vendors/biomyst-labs #### VANDL Labs 2 listings · from $34.99 https://www.bodyhackguide.co/vendors/vandl-labs #### Disguised Alpha 1 listing · from $64.99 https://www.bodyhackguide.co/vendors/disguised-alpha How we score these vendors Every supplier above is graded 0 to 100 on COA verification, payment transparency, shipping, reviews, and active listings. Methodology published, no pay-to-rank. View Scorecard (https://www.bodyhackguide.co/vendors/scorecard) ### Related Compounds View All (https://www.bodyhackguide.co/wiki) ### BPC-157 Injury, Repair & Recovery Preclinical BPC-157 (Body Protection Compound-157) is a synthetic pentadecapeptide consisting of 15 amino acids (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val) derived from a partial sequence of human gastric juice protein BPC. t½ Short plasma half-life. The first formal preclinical ADME study reported an elimination half-life under ~30 minutes after IV/IM dosing in rats and dogs, with linear dose-proportional kinetics (PMID: 36588717); a 2026 biopharmaceutical review confirms this sub-30-minute plasma half-life and highlights a pharmacokinetic-pharmacodynamic disconnect, as biological effects persist for hours to days (PMID: 42198317). No full human pharmacokinetic study has been published; twice-daily dosing is empirical/community-standard rather than PK-derived. 200-500 mcg subcutaneous 1-2x daily (community protocols); 1-10 mcg/kg in animal studies 214 PubMed View Profile https://www.bodyhackguide.co/compound/bpc-157 ### Side-by-Side Comparisons All Comparisons (https://www.bodyhackguide.co/compare) TB-500 vs BPC-157: https://www.bodyhackguide.co/compare/bpc-157-vs-tb-500 TB-500 vs Thymosin Alpha-1 (TA1): https://www.bodyhackguide.co/compare/tb-500-vs-thymosin-alpha-1 View Full Dosage Guide → Protocols, calculator & safety for TB-500 https://www.bodyhackguide.co/guides/dosage/tb-500 ### Related Articles All Posts (https://www.bodyhackguide.co/blog) #### The 8 Best Peptide Companies for Research: 2026 Purity & Price Comparison A data-driven comparison of every active peptide vendor we track: COA audit results, price-per-mg tables for 19 compounds, and a step-by-step purchase guide. 9/16/2026 https://www.bodyhackguide.co/blog/best-peptide-companies-2026 #### Aquasome Nanotechnology and Oral Peptides: The Mechanism, the Research, and the 10 Compounds It Actually Works For how aquasome 3-layer nano-encapsulation lets peptides like retatrutide, bpc-157, and tb-500 survive the gut. pubmed-backed deep dive on the 10 compounds it works for. 5/20/2026 https://www.bodyhackguide.co/blog/aqasome-nanotechnology-oral-peptides #### Tirzepatide to Retatrutide Switch Protocol: A Crossover Guide (2026) How to cross-titrate from tirzepatide (Mounjaro/Zepbound) to retatrutide safely. The 2-3 week hold + titration ladder, what bloodwork to track, when the switch makes sense, and when to stay on tirzepatide. 5/18/2026 https://www.bodyhackguide.co/blog/tirzepatide-to-retatrutide-switch-protocol ### Featured Price Ion Peptide $29.00($5.80/mg) 4 vendors · 8 listings ### Research Score 100 1,039 PubMed results ### Quality Indicators Data Completeness 100% Description Mechanism of Action Chemical Data Dosing Protocols Safety Profile PubMed Results Interactions Vendor Listings Research Volume 1,039 PubMed results Well-researched compound ### Quick Facts Half-Life Molecular Weight Administration Subcutaneous, Intramuscular CAS Number 77591-33-4 Trial Phase Phase 2 ### Safety Profile Common Side Effects - • Injection site discomfort (subcutaneous administration) - • Mild fatigue or lethargy in the days following initial doses - • Temporary head rush or flushing reported anecdotally Research Disclaimer This information is for educational and research purposes only. Not intended as medical advice. Consult a healthcare professional before use. The Wolverine Stack: BPC-157 + TB-500 Protocol Guide Complete synergistic recovery protocol: dosing tables, cycling, reconstitution, and 9 PubMed citations. https://www.bodyhackguide.co/blog/wolverine-stack-bpc-157-tb-500-protocol-guide ## Frequently Asked Questions What is TB-500 used for in research? TB-500 is the name the market and the FDA use for a short synthetic fragment of thymosin beta-4: the seven-amino-acid, N-acetylated actin-binding sequence Ac-LKKTETQ (residues 17 to 23). Thymosin beta-4 itself is a 43-amino-acid peptide (about 4963 Da acetylated) and one of the most abundant intracellular actin-sequestering molecules in mammalian cells. The two are not the same molecule. Analytical work on internet-sold TB-500 found products that did not match their descriptions (PMID: 36482504), and a 2024 metabolite study states that the biological effects of the acetylated TB-500 fragment have not been documented (PMID: 38382158). The sequence and mass on a lot certificate of analysis are the only way to know which form a vial holds. The foundational review by Goldstein et al. (2005) established full-length thymosin beta-4 as a multifunctional regenerative peptide with roles spanning wound healing, angiogenesis, anti-inflammation, and cardiac repair. Unlike many bioactive peptides, thymosin beta-4 is not a hormone or cytokine but rather an intracellular actin-regulatory protein that, when released extracellularly following injury, initiates paracrine signaling cascades that drive tissue repair. Most of what follows is full-length thymosin beta-4 data; the fragment's efficacy data appears only in the wound-healing paragraph. Thymosin beta-4 has been studied extensively in preclinical models of cardiac injury. Bock-Marquette et al. (2004) demonstrated that thymosin beta-4 promotes survival of cardiomyocytes after experimental myocardial infarction in mice, reduces infarct size, and improves cardiac function. The mechanism involves activation of the Akt (protein kinase B) survival pathway and migration of cardiomyocytes and endothelial cells toward the injury. These findings positioned thymosin beta-4 as a candidate cardiac repair agent, though clinical translation has been limited. In wound healing research, thymosin beta-4 accelerates dermal wound closure by promoting keratinocyte and endothelial cell migration, increasing angiogenesis, and reducing inflammation at the wound site. Philp et al. (2003) showed that topical thymosin beta-4 significantly accelerated full-thickness wound closure in diabetic and aged mouse models, and in the same paper the free seven-residue fragment LKKTETQ promoted repair in aged animals comparable to the parent peptide (PMID: 12581423). That one mouse study, plus in-vitro assays, is the fragment record; there is no human data for the fragment. Thymosin beta-4 has also shown efficacy in corneal wound healing models. Sosne et al. demonstrated that thymosin beta-4 eye drops accelerate corneal epithelial wound closure and reduce inflammation after chemical or mechanical injury, leading to ophthalmic clinical trials (Phase 2 and Phase 3, RGN-259) for dry eye and neurotrophic keratopathy. This is the most advanced clinical development program for any thymosin beta-4-based therapeutic, and it is topical. TB-500 is not FDA-approved for any human therapeutic indication. It is a doping-control target in racing horses (PMID: 23084823) and is used in the human peptide biohacking community for musculoskeletal recovery, wound healing, and anti-inflammatory effects, on the strength of the full-length peptide literature rather than any human fragment data. What forms does TB-500 come in? TB-500 is available in vial form. How much does TB-500 cost? Prices start at $29.00 across 4 vendors. How do I compare TB-500 vendors? Compare prices, payment methods, shipping, and COA scores across 4 vendors. ## Research Tools ### Peptide Calculator Reconstitution & syringe units https://www.bodyhackguide.co/tools/reconstitution ### Reconstitution Guide How to mix, step by step https://www.bodyhackguide.co/guides/how-to-reconstitute-peptides ### Nasal Spray Calc mL per actuation https://www.bodyhackguide.co/tools/intranasal ### Half-Life Visualizer Decay curves https://www.bodyhackguide.co/tools/halflife ## Related Compounds ### BPC-157 Injury, Repair & Recovery Preclinical ## Side-by-Side Comparisons Compare TB-500 head-to-head: mechanism, half-life, dosing, safety, and vendor prices. ## Research Benches Reference pages that put TB-500 next to the compounds people pair it with: roles, evidence, overlap and conflicts. No dosing. Research use only. Tissue Repair Bench KLOW, GHK-Cu, BPC-157, TB-500, KPV and ARA-290, one name at a time research bench · no dosing https://www.bodyhackguide.co/stack/tissue-repair-bench Free 2026 Peptide Cheat Sheet (PDF) 38 compounds and blends: vial sizes, BAC water volumes, dosing ranges and bloodwork markers. Download Free (https://www.bodyhackguide.co/guides/peptide-cheat-sheet-download) ### Need bloodwork before starting? Full hormone + metabolic panels from Anabolic Insights. Code CHONCH for first-order discount. Image: BodyHackGuide (https://www.bodyhackguide.co/assets/bodyhackguide-logo-DOzZNUyh.webp) ### BodyHackGuide Your biohacking research hub: compound pricing, brain health protocols, dosing tools, and vendor reviews. support@bodyhackguide.co Join our Discord: https://discord.gg/Mhq5UdRYBA r/BodyHackGuide on Reddit: https://reddit.com/r/BodyHackGuide #### Explore - Compare prices: https://www.bodyhackguide.co/compare - Vendors: https://www.bodyhackguide.co/vendors - Deals: https://www.bodyhackguide.co/deals - Coupons: https://www.bodyhackguide.co/coupons - Nootropics: https://www.bodyhackguide.co/nootropics - Brain health: https://www.bodyhackguide.co/brain-health - Blog: https://www.bodyhackguide.co/blog - Guides: https://www.bodyhackguide.co/guides #### Tools - Reconstitution: https://www.bodyhackguide.co/tools/reconstitution - Half-life visualizer: https://www.bodyhackguide.co/tools/halflife - COA reader: https://www.bodyhackguide.co/tools/coa - Cost calculator: https://www.bodyhackguide.co/tools/cost-calculator - Stack builder: https://www.bodyhackguide.co/stack-builder - All tools: https://www.bodyhackguide.co/tools #### Trust & vendors - Vendor directory: https://www.bodyhackguide.co/vendors - Trust scorecard: https://www.bodyhackguide.co/vendors/scorecard - Vendor trust badge: https://www.bodyhackguide.co/vendors/get-vetted - Scoring methodology: https://www.bodyhackguide.co/vendors/methodology - Peptide cheat sheet: https://www.bodyhackguide.co/guides/peptide-cheat-sheet-download #### Company - About: https://www.bodyhackguide.co/about - Affiliate disclosure: https://www.bodyhackguide.co/affiliate-disclosure - Editorial standards: https://www.bodyhackguide.co/editorial-standards - Creators: https://www.bodyhackguide.co/creators - Coaches: https://www.bodyhackguide.co/coaches - 1-on-1 coaching: https://www.bodyhackguide.co/coaching - Privacy: https://www.bodyhackguide.co/privacy - Terms: https://www.bodyhackguide.co/terms - Full site directory: https://www.bodyhackguide.co/site-directory **Affiliate Disclosure:** We rank every vendor on the same public, reproducible methodology (https://www.bodyhackguide.co/vendors/methodology), and we earn a commission from purchases made through some of our links at no extra cost to you. 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All rights reserved. · Terms (https://www.bodyhackguide.co/terms) · Privacy (https://www.bodyhackguide.co/privacy) ## Structured data ```json [ { "@context": "https://schema.org", "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Home", "item": "https://www.bodyhackguide.co/" }, { "@type": "ListItem", "position": 2, "name": "Compounds", "item": "https://www.bodyhackguide.co/compare" }, { "@type": "ListItem", "position": 3, "name": "TB-500" } ] }, { "@context": "https://schema.org", "@type": "Product", "name": "TB-500", "description": "TB-500 is the name the market and the FDA use for a short synthetic fragment of thymosin beta-4: the seven-amino-acid, N-acetylated actin-binding sequence Ac-LKKTETQ (residues 17 to 23). Thymosin beta-4 itself is a 43-amino-acid peptide (about 4963 Da acetylated) and one of the most abundant intracellular actin-sequestering molecules in mammalian cells. The two are not the same molecule. Analytical work on internet-sold TB-500 found products that did not match their descriptions (PMID: 36482504), and a 2024 metabolite study states that the biological effects of the acetylated TB-500 fragment have not been documented (PMID: 38382158). The sequence and mass on a lot certificate of analysis are the only way to know which form a vial holds. The foundational review by Goldstein et al. (2005) established full-length thymosin beta-4 as a multifunctional regenerative peptide with roles spanning wound healing, angiogenesis, anti-inflammation, and cardiac repair. Unlike many bioactive peptides, thymosin beta-4 is not a hormone or cytokine but rather an intracellular actin-regulatory protein that, when released extracellularly following injury, initiates paracrine signaling cascades that drive tissue repair. Most of what follows is full-length thymosin beta-4 data; the fragment's efficacy data appears only in the wound-healing paragraph. Thymosin beta-4 has been studied extensively in preclinical models of cardiac injury. Bock-Marquette et al. (2004) demonstrated that thymosin beta-4 promotes survival of cardiomyocytes after experimental myocardial infarction in mice, reduces infarct size, and improves cardiac function. The mechanism involves activation of the Akt (protein kinase B) survival pathway and migration of cardiomyocytes and endothelial cells toward the injury. These findings positioned thymosin beta-4 as a candidate cardiac repair agent, though clinical translation has been limited. In wound healing research, thymosin beta-4 accelerates dermal wound closure by promoting keratinocyte and endothelial cell migration, increasing angiogenesis, and reducing inflammation at the wound site. Philp et al. (2003) showed that topical thymosin beta-4 significantly accelerated full-thickness wound closure in diabetic and aged mouse models, and in the same paper the free seven-residue fragment LKKTETQ promoted repair in aged animals comparable to the parent peptide (PMID: 12581423). That one mouse study, plus in-vitro assays, is the fragment record; there is no human data for the fragment. Thymosin beta-4 has also shown efficacy in corneal wound healing models. Sosne et al. demonstrated that thymosin beta-4 eye drops accelerate corneal epithelial wound closure and reduce inflammation after chemical or mechanical injury, leading to ophthalmic clinical trials (Phase 2 and Phase 3, RGN-259) for dry eye and neurotrophic keratopathy. This is the most advanced clinical development program for any thymosin beta-4-based therapeutic, and it is topical. TB-500 is not FDA-approved for any human therapeutic indication. It is a doping-control target in racing horses (PMID: 23084823) and is used in the human peptide biohacking community for musculoskeletal recovery, wound healing, and anti-inflammatory effects, on the strength of the full-length peptide literature rather than any human fragment data.", "url": "https://www.bodyhackguide.co/compound/tb-500", "image": "https://www.bodyhackguide.co/og-image.png", "brand": { "@type": "Brand", "name": "Research Compound" }, "offers": { "@type": "AggregateOffer", "lowPrice": "29.00", "highPrice": "64.99", "priceCurrency": "USD", "offerCount": "7", "availability": "https://schema.org/InStock", "offers": [ { "@type": "Offer", "price": "29.00", "priceCurrency": "USD", "availability": "https://schema.org/InStock", "itemCondition": "https://schema.org/NewCondition", "url": "https://ionpeptide.com/product/tb-500/?ref=reddit15", "seller": { "@type": "Organization", "name": "Ion Peptide", "url": "https://ionpeptide.com" }, "name": "TB-500 5mg vial" }, { "@type": "Offer", "price": "34.99", "priceCurrency": "USD", "availability": "https://schema.org/InStock", "itemCondition": "https://schema.org/NewCondition", "url": "https://biomystlabs.com/product/tb500-5mg/?ref=rxtkqorwv", "seller": { "@type": "Organization", "name": "BioMyst Labs", "url": "https://biomystlabs.com" }, "name": "TB-500 5mg vial" }, { "@type": "Offer", "price": "34.99", "priceCurrency": "USD", "availability": "https://schema.org/InStock", "itemCondition": "https://schema.org/NewCondition", "url": "https://www.vandl-labs.com/product/tb-500/?ref=choncho", "seller": { "@type": "Organization", "name": "VANDL Labs", "url": "https://www.vandl-labs.com/?ref=choncho" }, "name": "TB-500 5mg vial" }, { "@type": "Offer", "price": "36.99", "priceCurrency": "USD", "availability": "https://schema.org/InStock", "itemCondition": "https://schema.org/NewCondition", "url": "https://biomystlabs.com/product/tb500-5mg/?ref=rxtkqorwv", "seller": { "@type": "Organization", "name": "BioMyst Labs", "url": "https://biomystlabs.com" }, "name": "TB-500 5mg vial" }, { "@type": "Offer", "price": "49.00", "priceCurrency": "USD", "availability": "https://schema.org/InStock", "itemCondition": "https://schema.org/NewCondition", "url": "https://ionpeptide.com/product/tb-500/?ref=reddit15", "seller": { "@type": "Organization", "name": "Ion Peptide", "url": "https://ionpeptide.com" }, "name": "TB-500 10mg vial" }, { "@type": "Offer", "price": "49.99", "priceCurrency": "USD", "availability": "https://schema.org/InStock", "itemCondition": "https://schema.org/NewCondition", "url": "https://www.vandl-labs.com/product/tb-500/?ref=choncho", "seller": { "@type": "Organization", "name": "VANDL Labs", "url": "https://www.vandl-labs.com/?ref=choncho" }, "name": "TB-500 10mg vial" }, { "@type": "Offer", "price": "64.99", "priceCurrency": "USD", "availability": "https://schema.org/InStock", "itemCondition": "https://schema.org/NewCondition", "url": "https://disguisedalpha.com/product/tb-4/?coupon=reddit", "seller": { "@type": "Organization", "name": "Disguised Alpha", "url": "https://disguisedalpha.com/?coupon=reddit" } } ] } }, { "@context": "https://schema.org", "@type": "MedicalWebPage", "name": "TB-500", "description": "TB-500 is the name the market and the FDA use for a short synthetic fragment of thymosin beta-4: the seven-amino-acid, N-acetylated actin-binding sequence Ac-LKKTETQ (residues 17 to 23). Thymosin beta-4 itself is a 43-amino-acid peptide (about 4963 Da acetylated) and one of the most abundant intracellular actin-sequestering molecules in mammalian cells. The two are not the same molecule. Analytical work on internet-sold TB-500 found products that did not match their descriptions (PMID: 36482504), and a 2024 metabolite study states that the biological effects of the acetylated TB-500 fragment have not been documented (PMID: 38382158). The sequence and mass on a lot certificate of analysis are the only way to know which form a vial holds. The foundational review by Goldstein et al. (2005) established full-length thymosin beta-4 as a multifunctional regenerative peptide with roles spanning wound healing, angiogenesis, anti-inflammation, and cardiac repair. Unlike many bioactive peptides, thymosin beta-4 is not a hormone or cytokine but rather an intracellular actin-regulatory protein that, when released extracellularly following injury, initiates paracrine signaling cascades that drive tissue repair. Most of what follows is full-length thymosin beta-4 data; the fragment's efficacy data appears only in the wound-healing paragraph. Thymosin beta-4 has been studied extensively in preclinical models of cardiac injury. Bock-Marquette et al. (2004) demonstrated that thymosin beta-4 promotes survival of cardiomyocytes after experimental myocardial infarction in mice, reduces infarct size, and improves cardiac function. The mechanism involves activation of the Akt (protein kinase B) survival pathway and migration of cardiomyocytes and endothelial cells toward the injury. These findings positioned thymosin beta-4 as a candidate cardiac repair agent, though clinical translation has been limited. In wound healing research, thymosin beta-4 accelerates dermal wound closure by promoting keratinocyte and endothelial cell migration, increasing angiogenesis, and reducing inflammation at the wound site. Philp et al. (2003) showed that topical thymosin beta-4 significantly accelerated full-thickness wound closure in diabetic and aged mouse models, and in the same paper the free seven-residue fragment LKKTETQ promoted repair in aged animals comparable to the parent peptide (PMID: 12581423). That one mouse study, plus in-vitro assays, is the fragment record; there is no human data for the fragment. Thymosin beta-4 has also shown efficacy in corneal wound healing models. Sosne et al. demonstrated that thymosin beta-4 eye drops accelerate corneal epithelial wound closure and reduce inflammation after chemical or mechanical injury, leading to ophthalmic clinical trials (Phase 2 and Phase 3, RGN-259) for dry eye and neurotrophic keratopathy. This is the most advanced clinical development program for any thymosin beta-4-based therapeutic, and it is topical. TB-500 is not FDA-approved for any human therapeutic indication. It is a doping-control target in racing horses (PMID: 23084823) and is used in the human peptide biohacking community for musculoskeletal recovery, wound healing, and anti-inflammatory effects, on the strength of the full-length peptide literature rather than any human fragment data.", "lastReviewed": "2026-05-04T11:35:35.257+00:00", "url": "https://www.bodyhackguide.co/compound/tb-500", "author": { "@type": "Person", "name": "BioChonch", "url": "https://www.bodyhackguide.co/about", "jobTitle": "Founder & Lead Researcher", "sameAs": [ "https://reddit.com/r/BodyHackGuide", "https://reddit.com/r/BrainHackGuide", "https://reddit.com/r/Biohackingher" ], "worksFor": { "@type": "Organization", "name": "BodyHackGuide", "url": "https://www.bodyhackguide.co" } }, "reviewedBy": { "@type": "Person", "name": "BioChonch", "url": "https://www.bodyhackguide.co/about", "jobTitle": "Founder & Lead Researcher", "sameAs": [ "https://reddit.com/r/BodyHackGuide", "https://reddit.com/r/BrainHackGuide", "https://reddit.com/r/Biohackingher" ], "worksFor": { "@type": "Organization", "name": "BodyHackGuide", "url": "https://www.bodyhackguide.co" } }, "publisher": { "@type": "Organization", "name": "BodyHackGuide", "url": "https://www.bodyhackguide.co" }, "mainContentOfPage": { "@type": "WebPageElement", "cssSelector": "main" }, "about": { "@type": "Drug", "name": "TB-500", "alternateName": [ "Thymosin Beta-4 fragment (17-23)", "TB4 fragment", "TB500", "TB 500", "Ac-LKKTETQ" ], "description": "TB-500 is the name the market and the FDA use for a short synthetic fragment of thymosin beta-4: the seven-amino-acid, N-acetylated actin-binding sequence Ac-LKKTETQ (residues 17 to 23). Thymosin beta-4 itself is a 43-amino-acid peptide (about 4963 Da acetylated) and one of the most abundant intracellular actin-sequestering molecules in mammalian cells. The two are not the same molecule. Analytical work on internet-sold TB-500 found products that did not match their descriptions (PMID: 36482504), and a 2024 metabolite study states that the biological effects of the acetylated TB-500 fragment have not been documented (PMID: 38382158). The sequence and mass on a lot certificate of analysis are the only way to know which form a vial holds. The foundational review by Goldstein et al. (2005) established full-length thymosin beta-4 as a multifunctional regenerative peptide with roles spanning wound healing, angiogenesis, anti-inflammation, and cardiac repair. Unlike many bioactive peptides, thymosin beta-4 is not a hormone or cytokine but rather an intracellular actin-regulatory protein that, when released extracellularly following injury, initiates paracrine signaling cascades that drive tissue repair. Most of what follows is full-length thymosin beta-4 data; the fragment's efficacy data appears only in the wound-healing paragraph. Thymosin beta-4 has been studied extensively in preclinical models of cardiac injury. Bock-Marquette et al. (2004) demonstrated that thymosin beta-4 promotes survival of cardiomyocytes after experimental myocardial infarction in mice, reduces infarct size, and improves cardiac function. The mechanism involves activation of the Akt (protein kinase B) survival pathway and migration of cardiomyocytes and endothelial cells toward the injury. These findings positioned thymosin beta-4 as a candidate cardiac repair agent, though clinical translation has been limited. In wound healing research, thymosin beta-4 accelerates dermal wound closure by promoting keratinocyte and endothelial cell migration, increasing angiogenesis, and reducing inflammation at the wound site. Philp et al. (2003) showed that topical thymosin beta-4 significantly accelerated full-thickness wound closure in diabetic and aged mouse models, and in the same paper the free seven-residue fragment LKKTETQ promoted repair in aged animals comparable to the parent peptide (PMID: 12581423). That one mouse study, plus in-vitro assays, is the fragment record; there is no human data for the fragment. Thymosin beta-4 has also shown efficacy in corneal wound healing models. Sosne et al. demonstrated that thymosin beta-4 eye drops accelerate corneal epithelial wound closure and reduce inflammation after chemical or mechanical injury, leading to ophthalmic clinical trials (Phase 2 and Phase 3, RGN-259) for dry eye and neurotrophic keratopathy. This is the most advanced clinical development program for any thymosin beta-4-based therapeutic, and it is topical. TB-500 is not FDA-approved for any human therapeutic indication. It is a doping-control target in racing horses (PMID: 23084823) and is used in the human peptide biohacking community for musculoskeletal recovery, wound healing, and anti-inflammatory effects, on the strength of the full-length peptide literature rather than any human fragment data.", "activeIngredient": "TB-500", "administrationRoute": "Subcutaneous, Intramuscular", "mechanismOfAction": "Actin Sequestration and Cytoskeletal Dynamics The primary molecular function of thymosin beta-4 (and the synthetic peptide sold as TB-500) is binding to monomeric G-actin in a 1:1 complex, preventing its polymerization into F-actin filaments (PMID: 1627561). By maintaining a pool of sequestered G-actin, thymosin beta-4 enables rapid cytoskeletal reorganization when cells need to migrate, divide, or change shape. This actin-sequestering function is critical during wound healing because it allows keratinocytes, endothelial cells, and fibroblasts to rapidly mobilize to injury sites. The actin-binding activity centers on the conserved LKKTETQ motif (residues 17-23, a 7-amino-acid sequence), an example of the beta-thymosin/WH2 actin-binding fold (PMID: 17468236); this heptapeptide lies within the full-length thymosin beta-4 molecule that TB-500 reproduces. Angiogenesis Promotion TB-500 promotes the formation of new blood vessels through multiple mechanisms. Thymosin beta-4 stimulates endothelial cell migration and tube formation in Matrigel assays, upregulates VEGF expression, and promotes vascular sprouting in ischemic tissues. In cardiac injury models, thymosin beta-4 treatment increased capillary density in the peri-infarct region, improving oxygen delivery to surviving cardiomyocytes and reducing subsequent fibrosis. The pro-angiogenic effect is mediated partly through activation of the integrin-linked kinase (ILK) and Akt survival pathways. Anti-Inflammatory Signaling Thymosin beta-4 suppresses inflammatory signaling through downregulation of NF-kB nuclear translocation and reduction of pro-inflammatory cytokine production (TNF-alpha, IL-1beta, IL-6). In wound healing models, TB-500-treated animals showed reduced neutrophil infiltration and earlier transition from the inflammatory phase to the proliferative phase of repair. This anti-inflammatory property is complementary to its pro-migratory effects, creating a tissue environment that favors regeneration over fibrotic scar formation. Cardiac Repair and Progenitor Cell Activation In the context of myocardial infarction, thymosin beta-4 activates epicardium-derived progenitor cells (EPDCs), stimulating them to differentiate into endothelial cells and smooth muscle cells that contribute to vascular regeneration. This mechanism involves upregulation of the transcription factor Wilms tumor 1 (WT1) and activation of the Akt/mTOR survival pathway in cardiomyocytes. In preclinical models, thymosin beta-4 reduces apoptosis of cardiomyocytes under ischemic stress by promoting Akt phosphorylation, which inhibits the pro-apoptotic protein Bad (PMID: 17600280).", "dosageForm": "vial", "legalStatus": "Not approved for human use (research chemical)", "warning": "For research purposes only. Not for human consumption." } }, { "@context": "https://schema.org", "@type": "FAQPage", "mainEntity": [ { "@type": "Question", "name": "What is TB-500 used for in research?", "acceptedAnswer": { "@type": "Answer", "text": "TB-500 is the name the market and the FDA use for a short synthetic fragment of thymosin beta-4: the seven-amino-acid, N-acetylated actin-binding sequence Ac-LKKTETQ (residues 17 to 23). Thymosin beta-4 itself is a 43-amino-acid peptide (about 4963 Da acetylated) and one of the most abundant intracellular actin-sequestering molecules in mammalian cells. The two are not the same molecule. Analytical work on internet-sold TB-500 found products that did not match their descriptions (PMID: 36482504), and a 2024 metabolite study states that the biological effects of the acetylated TB-500 fragment have not been documented (PMID: 38382158). The sequence and mass on a lot certificate of analysis are the only way to know which form a vial holds. The foundational review by Goldstein et al. (2005) established full-length thymosin beta-4 as a multifunctional regenerative peptide with roles spanning wound healing, angiogenesis, anti-inflammation, and cardiac repair. Unlike many bioactive peptides, thymosin beta-4 is not a hormone or cytokine but rather an intracellular actin-regulatory protein that, when released extracellularly following injury, initiates paracrine signaling cascades that drive tissue repair. Most of what follows is full-length thymosin beta-4 data; the fragment's efficacy data appears only in the wound-healing paragraph. Thymosin beta-4 has been studied extensively in preclinical models of cardiac injury. Bock-Marquette et al. (2004) demonstrated that thymosin beta-4 promotes survival of cardiomyocytes after experimental myocardial infarction in mice, reduces infarct size, and improves cardiac function. The mechanism involves activation of the Akt (protein kinase B) survival pathway and migration of cardiomyocytes and endothelial cells toward the injury. These findings positioned thymosin beta-4 as a candidate cardiac repair agent, though clinical translation has been limited. In wound healing research, thymosin beta-4 accelerates dermal wound closure by promoting keratinocyte and endothelial cell migration, increasing angiogenesis, and reducing inflammation at the wound site. Philp et al. (2003) showed that topical thymosin beta-4 significantly accelerated full-thickness wound closure in diabetic and aged mouse models, and in the same paper the free seven-residue fragment LKKTETQ promoted repair in aged animals comparable to the parent peptide (PMID: 12581423). That one mouse study, plus in-vitro assays, is the fragment record; there is no human data for the fragment. Thymosin beta-4 has also shown efficacy in corneal wound healing models. Sosne et al. demonstrated that thymosin beta-4 eye drops accelerate corneal epithelial wound closure and reduce inflammation after chemical or mechanical injury, leading to ophthalmic clinical trials (Phase 2 and Phase 3, RGN-259) for dry eye and neurotrophic keratopathy. This is the most advanced clinical development program for any thymosin beta-4-based therapeutic, and it is topical. TB-500 is not FDA-approved for any human therapeutic indication. It is a doping-control target in racing horses (PMID: 23084823) and is used in the human peptide biohacking community for musculoskeletal recovery, wound healing, and anti-inflammatory effects, on the strength of the full-length peptide literature rather than any human fragment data." } }, { "@type": "Question", "name": "What forms does TB-500 come in?", "acceptedAnswer": { "@type": "Answer", "text": "TB-500 is available in vial form." } }, { "@type": "Question", "name": "How much does TB-500 cost?", "acceptedAnswer": { "@type": "Answer", "text": "Prices start at $29.00 across 4 vendors." } }, { "@type": "Question", "name": "How do I compare TB-500 vendors?", "acceptedAnswer": { "@type": "Answer", "text": "Compare prices, payment methods, shipping, and COA scores across 4 vendors." } } ] }, { "@context": "https://schema.org", "@type": "Organization", "@id": "https://www.bodyhackguide.co#organization", "name": "BodyHackGuide", "alternateName": "BHG", "url": "https://www.bodyhackguide.co", "logo": { "@type": "ImageObject", "url": "https://www.bodyhackguide.co/logo.png", "width": 512, "height": 512 }, "description": "Evidence-based research reference for peptides and nootropics. 124+ compound profiles, interactive dosing calculators, vendor price comparisons, and trust-scored vendor reviews scored on a public, reproducible methodology applied to every vendor. 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